Automated Quantitative Live Cell Fluorescence Microscopy

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Automated quantitative live cell fluorescence microscopy.

Advances in microscopy automation and image analysis have given biologists the tools to attempt large scale systems-level experiments on biological systems using microscope image readout. Fluorescence microscopy has become a standard tool for assaying gene function in RNAi knockdown screens and protein localization studies in eukaryotic systems. Similar high throughput studies can be attempted ...

متن کامل

Quantitative Live Cell Fluorescence-microscopy Analysis of Fission Yeast

Several microscopy techniques are available today that can detect a specific protein within the cell. During the last decade live cell imaging using fluorochromes like Green Fluorescent Protein (GFP) directly attached to the protein of interest has become increasingly popular. Using GFP and similar fluorochromes the subcellular localisations and movements of proteins can be detected in a fluore...

متن کامل

Live-cell Imaging Using Fluorescence Microscopy

The ever increasing abundance of different fluorescent proteins and vital dyes that are available to researchers today make it possible to study a large variety of dynamic biological processes in live-cells [1, 2]. Many proteins of interest can be studied by expressing fluorescent fusion proteins in cells, either transiently by transfection or viral infection or permanently using transgenic ani...

متن کامل

Automated analysis of time-lapse fluorescence microscopy images: from live cell images to intracellular foci

MOTIVATION Complete, accurate and reproducible analysis of intracellular foci from fluorescence microscopy image sequences of live cells requires full automation of all processing steps involved: cell segmentation and tracking followed by foci segmentation and pattern analysis. Integrated systems for this purpose are lacking. RESULTS Extending our previous work in cell segmentation and tracki...

متن کامل

Quantitative Fluorescence Microscopy

Printed in the Netherlands " You have walked to the edge of the great mosaic of human knowledge. Up until now, you have been living in a world full of ideas and concepts that other people have set out for you. Now it is your turn. You get to design a piece of the mosaic and glue it down. It just has to fit with what else is there. And if you do a good job shaping your tile, it will be easier fo...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Cold Spring Harbor Perspectives in Biology

سال: 2010

ISSN: 1943-0264

DOI: 10.1101/cshperspect.a000455